Anti-Amyloid Precursor Protein antibody [DE2B4] (ab12266)

- Amyloid Precursor Protein antibody [MABDE2B4] (ab12266)

Immunohistochemistry of formaldehyde fixed brain tissue, after antigen-retrieval by formic acid treatment, for post-mortem diagnosis of Alzheimer s disease.

Western blot - Amyloid Precursor Protein antibody [DE2B4] (ab12266)

Immunoprecipitation and immunoblotting of soluble APP, resulting from the action of alpha-secretase on membrane-bound APP.

Lane 1: monkey brain soluble fraction
Lane 2: conditioned medium from HEK 293 cells immunoprecipitated with ab12266.

After SDS-PAGE and transfer, the nitrocellulose was immunoblotted with MABDE2B4 followed by alkaline phosphatase-conjugated anti-mouse secondary antibody, then developd with BCIP/NBT chromogenic substrate.

The upper line marks APP₇₅₁, the lower line marks APP₆₉₅.

- Amyloid Precursor Protein antibody [MABDE2B4] (ab12266)

A section from the temporal lobe of a patient who died with Alzheimer’s disease was pre-treated with formic acid for 10 minutes prior to reaction with mouse monoclonal antibody DE4B2 (ab12266) that recognises an epitope near the N-terminus of β-amyloid peptides.  Bound antibody was detected by reaction with alkaline phosphatase-conjugated goat ant mouse IgG followed by BCIP/NBT substrate.  Plaques in the neuropil have been circled in black. Amyloid deposits stained by DE2B4 (ab12266) can be seen around some blood vessels (*) but some are not stained (#)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [DE2B4] (ab12266)

IHC image of ab12266 staining in human Alzheimer brain (cerebral cortex) formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12266, at a 1 in 40 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.