Amthamine dihydrobromide, H2 agonist (ab120778)
Key features and details
- Selective H2 agonist
- CAS Number: 142457-00-9
- Purity: > 99%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
Amthamine dihydrobromide, H2 agonist -
製品の詳細
Selective H2 agonist -
生理活性の詳細
Selective H2 agonist (pKi = 5.2). Weak antagonist of H3 and displays no activity at H1. Increases gastric acid secretion. Additionally increases blood pressure.
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精製度
> 99% -
CAS 番号
142457-00-9 -
構造式
製品の特性
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体系名
2-Amino-4-methyl-5-thiazoleethanamine dihydrobromide -
分子量
319.06 -
分子式
C6H11N3S.2HBr -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 100 mM -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120778の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120778, Amthamine dihydrobromide, H2 agonist
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ab78079 staining PKA R2 (phospho S96) in HepG2 cells treated with amthamine dihydrobromide (ab120778), by ICC/IF. Increase of PKA R2 (phospho S96) expression correlates with increased concentration of amthamine dihydrobromide, as described in literature.
The cells were incubated at 37°C for 60 minutes in media containing different concentrations of ab120778 (amthamine dihydrobromide) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab78079 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (1)
ab120778 は 1 報の論文で使用されています。
- Ma Q et al. Histamine H2 receptor deficit in glutamatergic neurons contributes to the pathogenesis of schizophrenia. Proc Natl Acad Sci U S A 120:e2207003120 (2023). PubMed: 36812204