Anti-Amphiphysin 抗体 [EP2061Y] (ab76124)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2061Y] to Amphiphysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Amphiphysin antibody [EP2061Y]
Amphiphysin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2061Y] to Amphiphysin -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human, mouse and rat brain tissue lysates IHC-P: Human brain tissue.
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特記事項
The production method for this product has been changed from hybridoma to recombniant on 16th April 2024
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2061Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76124の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/20000 - 1/100000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
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WB
1/20000 - 1/100000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
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機能
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. -
組織特異性
Neurons, certain endocrine cell types and spermatocytes. -
配列類似性
Contains 1 BAR domain.
Contains 1 SH3 domain. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 273 Human
- Entrez Gene: 218038 Mouse
- Entrez Gene: 60668 Rat
- Omim: 600418 Human
- SwissProt: P49418 Human
- SwissProt: Q7TQF7 Mouse
- SwissProt: O08838 Rat
- Unigene: 592182 Human
see all -
別名
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
see all
画像
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All lanes : Anti-Amphiphysin antibody [EP2061Y] (ab76124) at 1/100000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted? -
ab76124 at 1/100 dilution, staining Amphiphysin in human brain by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab76124 は論文での使用が確認できていません。