Anti-AP-A 抗体 (ab36122)
Key features and details
- Goat polyclonal to AP-A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-AP-A antibody
AP-A 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to AP-A -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig -
免疫原
Synthetic peptide corresponding to Human AP-A (internal sequence).
Sequence:EQYQKTSLAQEKEK
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ポジティブ・コントロール
- Human testis lysate. Human kidney tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36122の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 3 - 5 µg/ml.
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WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 109 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature, Ando et al. PMID 11994381. |
特記事項 |
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IHC-P
Use a concentration of 3 - 5 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 109 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature, Ando et al. PMID 11994381. |
ターゲット情報
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機能
Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells. -
組織特異性
Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues. -
配列類似性
Belongs to the peptidase M1 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 504350 Cow
- Entrez Gene: 2028 Human
- Entrez Gene: 397080 Pig
- Omim: 138297 Human
- SwissProt: Q32LQ0 Cow
- SwissProt: Q07075 Human
- SwissProt: Q95334 Pig
- Unigene: 435765 Human
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別名
- Aminopeptidase A antibody
- AMPE_HUMAN antibody
- AP-A antibody
see all
画像
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Anti-AP-A antibody (ab36122) at 1 µg/ml + Human Testis Lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Primary incubated for 1 hour. -
ab36122 staining AP-A in rat kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked using 20% serum for 1 hour at 25°C and then incubated with ab36122 at 3µg/ml for 1 hour. The secondary used was an undiluted HRP conjugated polyclonal.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab36122 は 6 報の論文で使用されています。
- Han WQ et al. Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition. J Physiol 596:3603-3616 (2018). PubMed: 29863758
- Nakamura T et al. Excess of Aminopeptidase A in the Brain Elevates Blood Pressure via the Angiotensin II Type 1 and Bradykinin B2 Receptors without Dipsogenic Effect. Int J Hypertens 2017:3967595 (2017). PubMed: 28421141
- Dvash E et al. Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage. Nat Commun 6:10112 (2015). IHC ; Mouse . PubMed: 26656251
- Härdtner C et al. High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic ß-cells. Int J Mol Med 32:795-804 (2013). WB ; Rat . PubMed: 23942780
- Velez JC et al. Angiotensin I is largely converted to angiotensin (1-7) and angiotensin (2-10) by isolated rat glomeruli. Hypertension 53:790-7 (2009). PubMed: 19289651
- Velez JC et al. Characterization of renin-angiotensin system enzyme activities in cultured mouse podocytes. Am J Physiol Renal Physiol 293:F398-407 (2007). WB ; Mouse . PubMed: 17429035