Anti-Amelotin 抗体 (ab122312)
Key features and details
- Rabbit polyclonal to Amelotin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Amelotin antibody -
製品の詳細
Rabbit polyclonal to Amelotin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen, corresponding to amino acids 16-110 (SLPQLKPALG LPPTKLAPDQ GTLPNQQQSN QVFPSLSLIP LTQMLTLGPD LHLLNPAAGM TPGTQTHPLT LGGLNVQQQL HPHVLPIFVT QLGAQ) of Human Amelotin.
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ポジティブ・コントロール
- Human cerebellum tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122312の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P | (2) |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Could be a cell adhesion protein involved in the maturation of tooth enamel. -
配列類似性
Belongs to the amelotin family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 401138 Human
- Omim: 610912 Human
- SwissProt: Q6UX39 Human
- Unigene: 453069 Human
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別名
- Amelotin antibody
- Amtn antibody
- AMTN_HUMAN antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab122312 は 5 報の論文で使用されています。
- Rajapakse D et al. Amelotin is expressed in retinal pigment epithelium and localizes to hydroxyapatite deposits in dry age-related macular degeneration. Transl Res 219:45-62 (2020). PubMed: 32160961
- Yamazaki M et al. Transcriptional regulation of human amelotin gene by interleukin-1ß. FEBS Open Bio 8:974-985 (2018). PubMed: 29928577
- Noda K et al. IL-1ß and TNF-a regulate mouse amelotin gene transcription in gingival epithelial cells. J Oral Sci 60:388-398 (2018). PubMed: 30158339
- Nakayama Y et al. Proinflammatory cytokines induce amelotin transcription in human gingival fibroblasts. J Oral Sci 56:261-8 (2014). PubMed: 25500923
- Lee HS et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . PubMed: 24376796