Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
None
Sample
Human Tissue sections (PD patient brain sections, mesencephalon, 40 um)
Specification
PD patient brain sections, mesencephalon, 40 um
Permeabilization
Yes - 0.5% Triton-X 100
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in TBS with 0.5% Triton-X 100
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Biotinylated anti-rabbit IgG (H+L)
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Horse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400
Additional data
Additional Notes
4% PFA fixation. This antibody provides low background and good specific staining of Lewy bodies and Lewy neurites (was compared to clone LB 509).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Oldriska Chutna
Verified customer
投稿 May 30 2014