Anti-Alpha-synuclein (phospho S129) 抗体 [EP1536Y] - BSA and Azide free (ab209422)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129) - BSA and Azide free
- Suitable for: IHC-P, ELISA, WB, Dot blot, IHC-FrFl
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] - BSA and Azide free
Alpha-synuclein 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1536Y] to Alpha-synuclein (phospho S129) - BSA and Azide free -
由来種
Rabbit -
特異性
This antibody only detects alpha synuclein phosphorylated on Ser129. IHC-P: This antibody showed no staining in human hippocampus normal brain and showed staining in Parkinson's brain as expected.
Mouse and rat species are recommended based on WB results, we do not guarantee IHC-FrFl and IHC-P for Mouse and rat.
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アプリケーション
適用あり: IHC-P, ELISA, WB, Dot blot, IHC-FrFlmore details
適用なし: Flow Cyt,IHC-Fr or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188826) -
ポジティブ・コントロール
- Fetal brain lysate.
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特記事項
ab209422 is the carrier-free version of ab51253.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1536Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 594 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab282652)
- Alexa Fluor® 488 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab310949)
- Alexa Fluor® 647 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab311067)
- Alexa Fluor® 555 Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab313137)
- Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253)
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab209422の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826).
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Dot blot |
Use at an assay dependent concentration.
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IHC-FrFl | (1) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).Can be blocked with Alpha-synuclein (phospho S129) peptide (ab188826). |
Dot blot
Use at an assay dependent concentration. |
IHC-FrFl
Use at an assay dependent concentration. |
ターゲット情報
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機能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
組織特異性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
関連疾患
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
配列類似性
Belongs to the synuclein family. -
ドメイン
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻訳後修飾
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
細胞内局在
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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参照データベース
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 21374 Human
see all -
別名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
画像
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All lanes : Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253) at 1/1000 dilution
Lane 1 : Mouse brain with Alzheimer’s disease tissue lysate
Lane 2 : Mouse brain with Alzheimer’s disease tissue lysate, membrane was incubated with alkaline phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 100,18 kDa why is the actual band size different from the predicted?
Exposure time: 140 secondsThis data was developed using ab51253, the same antibody clone in a different buffer.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Band around 100kda corresponds to αS oligomer (PMID: 27637918, PMID: 12597857)
Lysates used here were prepared using RIPA method. We recommend 1% SDS hot lysate method to reduce the detection of oligomers. Please refer here.
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This IHC data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).
IHC image of alpha Synuclein (phospho S129) staining in Human Parkinson Substantia Nigra formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51253, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay performed on Human normal Substantia Nigra.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Dot blot analysis of alpha Synuclein (pS129) peptide (Lane 1), alpha Synuclein (unmodified) peptide (Lane 2) labelling alpha Synuclein (pS129) with ab51253 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51253).
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This ELISA data was generated using the same anti-phospho alpha synuclein S129 antibody clone, EP1536Y, in a different buffer formulation (cat# ab51253).
Direct ELISA antibody dose-response curve using ab51253. Antibody concentration of 0-5000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (19)
ab209422 は 19 報の論文で使用されています。
- Martinez-Valbuena I et al. Mixed pathologies in pancreatic ß cells from subjects with neurodegenerative diseases and their interaction with prion protein. Acta Neuropathol Commun 9:64 (2021). PubMed: 33832546
- Martinez-Valbuena I et al. Interaction of amyloidogenic proteins in pancreatic ß cells from subjects with synucleinopathies. Acta Neuropathol N/A:N/A (2018). IHC-P ; Human . PubMed: 29536165
- Sasaki A et al. Sensitive western blotting for detection of endogenous Ser129-phosphorylated a-synuclein in intracellular and extracellular spaces. Sci Rep 5:14211 (2015). WB ; Human . PubMed: 26381815
- Gispert S et al. Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression. Hum Mol Genet 24:1061-76 (2015). WB, IHC-P, IHC-FrFl ; Mouse . PubMed: 25296918
- Wang S et al. Phosphatidylethanolamine deficiency disrupts a-synuclein homeostasis in yeast and worm models of Parkinson disease. Proc Natl Acad Sci U S A 111:E3976-85 (2014). PubMed: 25201965