Application
Western blot
Sample
Human Cell lysate - whole cell (Neuroblastoma)
Loading amount
15 µg
Specification
Neuroblastoma
Gel Running Conditions
Reduced Denaturing
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50%
Other product details
Dilution
1/1000
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: odyssey blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: odyssey Secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/15000
Detection
Detection method
Licor Odyssey
Exposure
1 second(s)
Bands
Specific: 16 kDa
Positive control
Lane three: M17 neuroblastoma overexpressing human alpha synuclein
Negative control
Lane one: Wild-type mouse brain
Lane two: SNCA knockout mouse brain
Lane two: SNCA knockout mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 16 2013