Anti-Cleaved NEAS (Asp1185) 抗体 [EPR3830-17] (ab92373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17]
Cleaved NEAS (Asp1185) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) -
由来種
Rabbit -
特異性
Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Cleaved NEAS (Asp1185) aa 1150-1250. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3830-17 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 285 kDa.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 285 kDa. |
ターゲット情報
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機能
Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. -
関連疾患
Epileptic encephalopathy, early infantile, 5 -
配列類似性
Belongs to the spectrin family.
Contains 3 EF-hand domains.
Contains 1 SH3 domain.
Contains 23 spectrin repeats. -
翻訳後修飾
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro. -
細胞内局在
Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6709 Human
- SwissProt: Q13813 Human
- Unigene: 372331 Human
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別名
- Alpha-II spectrin antibody
- Fodrin alpha chain antibody
- non-erythrocytic 1 antibody
see all
画像
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All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Lane 1 : HeLa + Camptothecin cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat + Staurosporine cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab92373 は論文での使用が確認できていません。