Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
40 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 21°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~270 kDa
Additional data
Additional Notes
An excellent antibody for immunoblotting. Gave a single species of the expected mobility.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jul 11 2007