Application
Western blot
Sample
Escherichia coli Recombinant protein (Mach1)
Loading amount
0.5 µg
Specification
Mach1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/8000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/16000
Detection
Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: 90 kDa
Additional data
Additional Notes
* The two lanes are the same samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 19 2010