Anti-Alcohol Dehydrogenase 抗体 [EPR4440] (ab108197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4440] to Alcohol Dehydrogenase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Alcohol Dehydrogenase antibody [EPR4440]
Alcohol Dehydrogenase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4440] to Alcohol Dehydrogenase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal liver, rat liver and mouse liver lysates IHC-P: Human liver tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4440 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes. |
ターゲット情報
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配列類似性
Belongs to the zinc-containing alcohol dehydrogenase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 124 Human
- Entrez Gene: 11522 Mouse
- Entrez Gene: 24172 Rat
- Omim: 103700 Human
- SwissProt: P07327 Human
- SwissProt: P00329 Mouse
- SwissProt: P06757 Rat
- Unigene: 654433 Human
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別名
- ADH alpha subunit antibody
- ADH antibody
- ADH1 antibody
see all
画像
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All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Mouse liver lysate
Lane 3 : Rat liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
Immunohistochemical staining of Alcohol Dehydrogenase in paraffin embedded Human liver tissue, using ab108197 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108197 は 1 報の論文で使用されています。
- Mouton AJ et al. Alcohol Vapor Inhalation as a Model of Alcohol-Induced Organ Disease. Alcohol Clin Exp Res 40:1671-8 (2016). PubMed: 27375174