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RabMAb

Anti-Alcohol Dehydrogenase 抗体 [EPR4439] (HRP) (ab202951)

製品の概要

  • 製品名
    Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP)
    Alcohol Dehydrogenase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4439] to Alcohol Dehydrogenase (HRP)
  • 由来種
    Rabbit
  • 標識
    HRP
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human Alcohol Dehydrogenase aa 1-100. The exact sequence is proprietary.
    Database link: P07327

  • ポジティブ・コントロール
    • WB: Mouse liver, Human fetal liver and Rat liver tissue lysates. IHC-P: FFPE normal human liver tissue sections.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab202951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 37,40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/125. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • IHC image of Alcohol Dehydrogenase staining in a section of formalin-fixed paraffin-embedded normal human liver tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins.  The section was then incubated with ab202951, 1/125 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4439] (HRP) (ab202951) at 1/5000 dilution

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 37,40 kDa (why is the actual band size different from the predicted?)


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab202951 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

     

参考文献

ab202951 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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