Application
Western blot
Sample
Schmidtea mediterranea Tissue lysate - whole (many adult somatic stem cells)
Loading amount
21 µg
Specification
many adult somatic stem cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 80 kDa
Negative control
Ago1 RNAi
Additional data
Additional Notes
the marker we used is fermentas SM#0671.the first lane is wildtype sample near the marker,and the second lane is the sample of expressing shRNA for Ago1.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. An Zeng
Verified customer
投稿 Mar 12 2010