Anti-Adipose Triglyceride Lipase (phospho S406) 抗体 (ab135093)
Key features and details
- Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406)
- Suitable for: ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Adipose Triglyceride Lipase (phospho S406) antibody
Adipose Triglyceride Lipase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Adipose Triglyceride Lipase (phospho S406) -
由来種
Rabbit -
特異性
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Dog, Pig, Chinese hamster -
免疫原
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ポジティブ・コントロール
- This antibody gave a positive signal in Mouse Brown Adipose Tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab135093の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. -
組織特異性
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). -
パスウェイ
Glycerolipid metabolism; triacylglycerol degradation. -
関連疾患
Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells. -
配列類似性
Contains 1 patatin domain. -
発生段階
Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium. -
細胞内局在
Lipid droplet. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 66853 Mouse
- Entrez Gene: 361676 Rat
- SwissProt: Q8BJ56 Mouse
- SwissProt: P0C548 Rat
- Unigene: 29998 Mouse
- Unigene: 19196 Rat
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別名
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
see all
画像
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All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/ml
Lane 1 : Mouse Brown Adipose Tissue (BAT)
Lane 2 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 412) (Phosphorylation S 406) at 1 µg/ml
Lane 3 : Mouse Brown Adipose Tissue (BAT) with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 413) (No Modifications) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 35 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 20 minutes -
ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab135093 は 18 報の論文で使用されています。
- Lyu K et al. Short-term overnutrition induces white adipose tissue insulin resistance through sn-1,2-diacylglycerol/PKCe/insulin receptor Thr1160 phosphorylation. JCI Insight 6:N/A (2021). PubMed: 33411692
- Qi G et al. ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism. Cell Rep 34:108572 (2021). PubMed: 33406436
- Molinari F et al. SIRT5 Inhibition Induces Brown Fat-Like Phenotype in 3T3-L1 Preadipocytes. Cells 10:N/A (2021). PubMed: 34066961
- Chen S et al. The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice. Front Physiol 12:709135 (2021). PubMed: 34588991
- Tarabra E et al. CIDEA expression in SAT from adolescent girls with obesity and unfavorable patterns of abdominal fat distribution. Obesity (Silver Spring) 29:2068-2080 (2021). PubMed: 34672413