Anti-Adiponectin 抗体 (ab62551)
Key features and details
- Rabbit polyclonal to Adiponectin
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Adiponectin antibody
Adiponectin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Adiponectin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
A 15 amino acid peptide from near the amino terminus of human Adiponectin.
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ポジティブ・コントロール
- Rat brain cell lysate. Rat brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62551の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 26 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
組織特異性
Synthesized exclusively by adipocytes and secreted into plasma. -
関連疾患
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
ドメイン
The C1q domain is commonly called the globular domain. -
翻訳後修飾
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
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別名
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
画像
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Lane 1 : Anti-Adiponectin antibody (ab62551) at 1 µg/ml
Lane 2 : Anti-Adiponectin antibody (ab62551) at 2 µg/ml
Lane 3 : Anti-Adiponectin antibody (ab62551) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted? -
ab62551, at 1µg/ml, staining Adiponectin in rat brain tissue by immunohistochemistry using paraffin-embedded tissue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab62551 は 15 報の論文で使用されています。
- Zhang YQ et al. Hyperlipidemic plasma molecules bind and inhibit adiponectin activity. J Diabetes Investig 13:947-954 (2022). PubMed: 35023319
- Hsu MH et al. Effects of Maternal Resveratrol on Maternal High-Fat Diet/Obesity with or without Postnatal High-Fat Diet. Int J Mol Sci 21:N/A (2020). PubMed: 32408716
- Leffler KE & Abdel-Rahman AA Restoration of Adiponectin-Connexin43 Signaling Mitigates Myocardial Inflammation and Dysfunction in Diabetic Female Rats. J Cardiovasc Pharmacol 75:259-267 (2020). PubMed: 31868825
- Jin F et al. DNMT1-mediated methylation inhibits microRNA-214-3p and promotes hair follicle stem cell differentiate into adipogenic lineages. Stem Cell Res Ther 11:444 (2020). PubMed: 33076979
- Tura A et al. Lower Levels of Adiponectin and Its Receptor Adipor1 in the Uveal Melanomas With Monosomy-3. Invest Ophthalmol Vis Sci 61:12 (2020). PubMed: 32396633