Anti-Adiponectin 抗体 - C-terminal (ab181699)
Key features and details
- Rabbit polyclonal to Adiponectin - C-terminal
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Adiponectin antibody - C-terminal
Adiponectin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Adiponectin - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Synthetic peptide corresponding to Human Adiponectin aa 224-244 (C terminal).
Sequence:LYADNDNDSTFTGFLLYHDTN
Database link: Q15848 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.
The detection limit for Adiponectin is approximately 1ng/lane under reducing conditions. |
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa. The detection limit for Adiponectin is approximately 1ng/lane under reducing conditions. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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機能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
組織特異性
Synthesized exclusively by adipocytes and secreted into plasma. -
関連疾患
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
ドメイン
The C1q domain is commonly called the globular domain. -
翻訳後修飾
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 282865 Cow
- Entrez Gene: 9370 Human
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- Omim: 605441 Human
- SwissProt: Q3Y5Z3 Cow
- SwissProt: Q15848 Human
- SwissProt: Q60994 Mouse
see all -
別名
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
画像
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Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml + rat heart tissue lysate
Predicted band size: 26 kDa -
All lanes : Anti-Adiponectin antibody - C-terminal (ab181699) at 0.5 µg/ml
Lane 1 : Recombinant Human Adiponectin protein at 0.01 µg
Lane 2 : Recombinant Human Adiponectin protein at 0.05 µg
Lane 3 : Recombinant Human Adiponectin protein at 0.0025 µg
Predicted band size: 26 kDa -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
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Immunohistochemical analysis of frozen rat liver tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Adiponectin using ab181699 at 1 μg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab181699 は 4 報の論文で使用されています。
- Droujinine IA et al. Proteomics of protein trafficking by in vivo tissue-specific labeling. Nat Commun 12:2382 (2021). PubMed: 33888706
- Zhang W et al. Forkhead box P1 (Foxp1) in osteoblasts regulates bone mass accrual and adipose tissue energy metabolism. J Bone Miner Res 36:2017-2026 (2021). PubMed: 34131944
- Shen B et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass. Elife 8:N/A (2019). PubMed: 30632962
- Yang T et al. Wogonin enhances intracellular adiponectin levels and suppresses adiponectin secretion in 3T3-L1 adipocytes. Endocr J 64:15-26 (2017). PubMed: 27667474