Anti-Adenosine A2b Receptor/ADORA2B 抗体 (ab229671)
Key features and details
- Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Adenosine A2b Receptor/ADORA2B antibody -
製品の詳細
Rabbit polyclonal to Adenosine A2b Receptor/ADORA2B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Adenosine A2b Receptor/ADORA2B (internal sequence). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P29275 -
ポジティブ・コントロール
- WB: U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. Mouse and rat brain tissue lysates. IHC-P: Mouse and rat brain tissues.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab229671の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB |
1/500 - 1/3000. Predicted molecular weight: 36 kDa.
|
特記事項 |
---|
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 36 kDa. |
ターゲット情報
-
機能
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 136 Human
- Entrez Gene: 11541 Mouse
- Entrez Gene: 29316 Rat
- Omim: 600446 Human
- SwissProt: P29275 Human
- SwissProt: Q60614 Mouse
- SwissProt: P29276 Rat
- Unigene: 167046 Human
see all -
別名
- AA2BR_HUMAN antibody
- Adenosine receptor A2b antibody
- adora 2 antibody
see all
画像
-
All lanes : Anti-Adenosine A2b Receptor/ADORA2B antibody (ab229671) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10% SDS-PAGE gel.
-
Paraffin-embedded mouse brain tissue stained for Adenosine A2b Receptor/ADORA2B using ab229671 at 1/500 dilution in immunohistochemical analysis.
-
All lanes : Anti-Adenosine A2b Receptor/ADORA2B antibody (ab229671) at 1/3000 dilution
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 36 kDa -
Paraffin-embedded rat brain tissue stained for Adenosine A2b Receptor/ADORA2B using ab229671 at 1/500 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab229671 は 1 報の論文で使用されています。
- Jin H et al. Increased Extracellular Adenosine in Radiotherapy-Resistant Breast Cancer Cells Enhances Tumor Progression through A2AR-Akt-ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33925516