Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-20% or 10%)
Sample
Human Cell lysate - whole cell (CHO-cells)
Specification
CHO-cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Bands
Specific: 37 kDa Non-specific: 60 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
CHO-cell line #1
Positive control
CHO-AR, line #2
Additional data
Abcam response
Thank you so much for testing this antibody in chinese hamster. We appreciate very much your efforts, but are sorry to hear that the antibody did not work in this species. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. David Wilkes
Verified customer
投稿 May 11 2012