Application
Western blot
Sample
Rhesus monkey Cell lysate - whole cell (PBMC)
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine)
Loading amount
30 µg
Treatment
1µg/mL E. coli LPS for 24hr (10^6 cells/mL)
Specification
PBMC
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 5% BSA
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Amersham ECL
Negative control
PBMC without LPS stimulation running buffer only
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 56 kDa Non-specific: 70, 75, 110 kDa
Positive control
Human PBMC stimulated with LPS to elicit Activin-A expression
Additional data
Additional Notes
Lane 1 Human PBMC cultured for 24 with LPS
Lane 2 Human PBMC cultured for 24 hours without LPS
Lane 3 Rhesus macaque PBMC cultured for 24 hours with LPS
Lane 4 Rhesus macaque PBMC cultured for 24 hours without LPS
Lane 5 Protein Ladder
WB PVDF Membrane
Cells lysed with SDS Lysing buffer + DDT and boiled 5 min at 95C immediately before run.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Alan Curtis
Verified customer
投稿 Jul 26 2016