Anti-Acinus 抗体 (ab7352)
Key features and details
- Rabbit polyclonal to Acinus
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Acinus antibody -
製品の詳細
Rabbit polyclonal to Acinus -
由来種
Rabbit -
特異性
The antibody recognises three different splice forms of Acinus, designated Acinus L, Acinus S and Acinus S'. The epitope is a region near the C-terminus of the cleaved active peptide p17. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
TRTALHGVKWPQSNPK
, corresponding to amino acids 1065-1080 of Human Acinus or 307 to 322 of human AcinusS, which are identical to those of mouse Acinus (2). The selected antigenic sequence is locatednear the C-terminus of the cleaved active peptide p17. -
ポジティブ・コントロール
- K562 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified IgG was prepared from whole serum by affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367).
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. Detects a band of approximately 220 kDa (predicted molecular weight: 151 kDa).Can be blocked with Acinus peptide (ab8367). |
ターゲット情報
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機能
Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3. -
組織特異性
Ubiquitous. -
配列類似性
Contains 1 SAP domain. -
翻訳後修飾
Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 22985 Human
- Omim: 604562 Human
- SwissProt: Q9UKV3 Human
- Unigene: 124490 Human
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別名
- ACIN 1 antibody
- ACIN1 antibody
- ACINU_HUMAN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence of K562 cells staining Acinus with ab7352 at 20 µg/ml
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ab7352 at 10µg/ml staining Acinus in K562 cells by ICC/IF
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Anti-Acinus antibody (ab7352) at 1 µg/ml + K562 whole cell lysate
Predicted band size: 151 kDa -
Immunofluorescence of Acinus in Hela cells using ab7352 at 10 ug/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab7352 は論文での使用が確認できていません。