Application
Western blot
Sample
Sheep Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine Gels)
Loading amount
75 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Non-Fat Dry Milk
Dilution
1/250
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 220 kDa Non-specific: 60 kDa
Additional data
Additional Notes
0.4 um Nitrocellulose membrane used Laemmli lysis buffer used in sample preparation and protease inhibitor cocktail.
Work performed by Adel Ghnenis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. John Odhiambo
Verified customer
投稿 Oct 14 2015