Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3-L1 mature adipocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
15 µg
Treatment
0.1% DMSO, AICAR 2mM
Specification
3T3-L1 mature adipocytes
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/4000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: LiCor Anti-Rabbit
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Detection
Detection method
Fluorescense
Negative control
Dorsomorphin 10uM, an established AMPK inhibitor.
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 250 kDa
Positive control
AICAR 2mM an established pAMPK activator that phosphorylates ACC.
Additional data
Additional Notes
PVDF membrane used. 4-12% Bis-Tris Invitrogen gel used. Protease and phosphotase inhibitors used.
Marker: 250kDa.
Lane 1: 0.1% DMSO.
Lane 2: Dorso 10uM.
Lane 3: AICAR 2mM.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Janaiya Samuels
Verified customer
投稿 Apr 27 2018