Anti-ACSS2 抗体 [EPR8500] (ab133664)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8500] to ACSS2
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ACSS2 antibody [EPR8500]
ACSS2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8500] to ACSS2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Wild-type HAP1, U-87 MG, HepG2, Caco 2 and fetal liver lysates, Mouse and Rat brain lysate; ICC/IF: Caco 2 and HepG2 cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8500 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133664の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100 - 1/250.
|
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
|
特記事項 |
---|
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 79 kDa. |
ターゲット情報
-
機能
Activates acetate so that it can be used for lipid synthesis or for energy generation. -
配列類似性
Belongs to the ATP-dependent AMP-binding enzyme family. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 55902 Human
- Entrez Gene: 60525 Mouse
- Entrez Gene: 311569 Rat
- Omim: 605832 Human
- SwissProt: Q9NR19 Human
- SwissProt: Q8BK97 Mouse
- SwissProt: Q9QXG4 Mouse
- SwissProt: D3ZFF9 Rat
see all -
別名
- ACAS2 antibody
- AceCS antibody
- Acetate CoA ligase antibody
see all
画像
-
All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate at 40 µg
Lane 2 : ACSS2 knockout HAP1 cell lysate at 40 µg
Lane 3 : HepG2 cell lysate at 20 µg
Lane 4 : U-87 MG cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 79 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ACSS2 antibody [EPR8500] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133664 was shown to bind specifically to ACSS2. A band was observed at 75 kDa in wild-type HAP1 cell lysates with no signal observed at this size in ACSS2 knockout cell line HAP1. To generate this image, wild-type and ACSS2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat brain lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 79 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling ACSS2 with purified ab133664 at 1:200 dilution (8.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
All lanes : Anti-ACSS2 antibody [EPR8500] (ab133664) at 1/1000 dilution (Unpurified)
Lane 1 : U87 MG lysate
Lane 2 : HepG2 lysate
Lane 3 : Caco 2 lysate
Lane 4 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa -
Immunofluorescence analysis of ACSS2 in Caco 2 cells labelled with unpurified ab133664 at a 1/100 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab133664 は 4 報の論文で使用されています。
- He W et al. MSC-regulated lncRNA MACC1-AS1 promotes stemness and chemoresistance through fatty acid oxidation in gastric cancer. Oncogene 38:4637-4654 (2019). PubMed: 30742067
- Gao X et al. Acetate functions as an epigenetic metabolite to promote lipid synthesis under hypoxia. Nat Commun 7:11960 (2016). IHC . PubMed: 27357947
- Mahmood S et al. Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway. Am J Physiol Endocrinol Metab 311:E117-27 (2016). PubMed: 27166281
- Soliman ML et al. Acetate reduces microglia inflammatory signaling in vitro. J Neurochem 123:555-67 (2012). WB ; Mouse . PubMed: 22924711