AcV5 tag antibody [AcV5] (ab49581)

Product Name 

AcV5 tag antibody [AcV5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [AcV5] to AcV5 tag

Immunogen 

AcNPV extracellular nonocculeded virus (NOV)

Species Reactivity 

Reacts with Autographa californica.
Not yet tested in other species.

Specificity 

This antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein. Proteins tagged with this AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.

Tested applications 
(see key)

Dot, ICC, IP

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at a concentration of 0.5 - 1 µg/ml.
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Extracts of Sf9 cells infected with baculovirus.

Research areas 

Tags & Cell Markers >> Epitope Tags >> V5 Tag

Relevance 

Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein.

AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp).

GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

AcV5

Myeloma 

Sp2/0-Ag14

Isotype 

IgG2b

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.400 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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