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Products:Tags & Cell Markers >> Epitope Tags >> V5 Tag
Overview
Product name
Anti-AcV5 tag antibody [AcV5]
AcV5 tag 抗体 (2件) 一覧
Description
Mouse monoclonal [AcV5] to AcV5 tag
Specificity
This antibody recognizes a nine amino acid residue tag (SWKDASGWS) of the baculovirus Autographa californica GP64 envelope fusion protein. Proteins tagged with this AcV5 peptide are functional in both dicots and monocots plants and show no cross reactivity across many plant species.
Tested applications
IP, ICC, Dot Blotmore details
Cross reactivity
Reacts with <i>Autographa californica</i>. Not yet tested in other species.
Immunogen
AcNPV extracellular nonocculeded virus (NOV)
Positive control
Extracts of Sf9 cells infected with baculovirus.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
AcV5
Myeloma
Sp2/0-Ag14
Isotype
IgG2b
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Dot: Use a concentration of 0.5 - 1 µg/ml.
Target
Relevance
Epitope tagged fusion proteins such as AcV5 tag are widely used in protein analysis of biological samples (e.g. plant cell extracts). Antibodies specific for the tag are a valuable means for the detection of the protein. AcV5 recognizes a nine amino acid residue tag of baculovirus Autographa californica GP64 envelope fusion protein (efp). GP64 gene open reading frame encodes a protein of 509 amino acids with a molecular weight of 58 kDa. The protein contains N-linked glycosylation sites and hydrophobic N- and C-termini, characteristic of signal and membrane anchor motifs found in envelope glycoproteins. The GP64 protein is present early (6 hr) post infection and accumulates in the infected cell moving to the periphery later.
References for Anti-AcV5 tag antibody [AcV5] (ab49581)
ab49581 has not yet been referenced specifically in any publications.
Publishing research using ab49581? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"