Application
Western blot
Sample
Human Cell lysate - nuclear (K562 erythroleukemia cell line)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
10000 cells
Specification
K562 erythroleukemia cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST, 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG Antibody, HRP-conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Two shRNAs against ATRX (90 and 92)
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 280, ~190, ~150, ~140, ~ kDa Non-specific: 64 kDa
Positive control
Control shRNA against luciferase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
David Valle-Garcia
Verified customer
投稿 May 09 2016