Alexa Fluor® 488 Anti-ATPase Inhibitory Factor 1/IF1 抗体 [5E2D7] (ab198075)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Alexa Fluor® 488 Anti-ATPase Inhibitory Factor 1/IF1 antibody [5E2D7]
ATPase Inhibitory Factor 1/IF1 一次抗体 製品一覧 -
製品の詳細
Alexa Fluor® 488 Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1/IF1 -
由来種
Mouse -
標識
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant full length protein corresponding to Rat ATPase Inhibitory Factor 1/IF1.
-
ポジティブ・コントロール
- ICC/IF: HepG2 cells.
-
特記事項
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
5E2D7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab198075の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min). |
特記事項 |
---|
ICC/IF
1/100. This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min). |
ターゲット情報
-
機能
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. -
配列類似性
Belongs to the ATPase inhibitor family. -
翻訳後修飾
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid. -
細胞内局在
Mitochondrion. - Information by UniProt
-
参照データベース
- Entrez Gene: 327699 Cow
- Entrez Gene: 93974 Human
- Entrez Gene: 11983 Mouse
- Entrez Gene: 25392 Rat
- SwissProt: P01096 Cow
- SwissProt: Q9UII2 Human
- SwissProt: O35143 Mouse
- SwissProt: Q03344 Rat
see all -
別名
- ATIF1_HUMAN antibody
- ATP synthase inhibitor protein antibody
- ATPase inhibitor antibody
see all
画像
-
ab198075 staining ATPase Inhibitory Factor 1/IF1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198075 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab198075 は論文での使用が確認できていません。