Anti-ATPase Inhibitory Factor 1 抗体 [5E2D7] (Alexa Fluor® 488) (ab198075)

製品の概要

  • 製品名
    Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (Alexa Fluor® 488)
    ATPase Inhibitory Factor 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5E2D7] to ATPase Inhibitory Factor 1 (Alexa Fluor® 488)
  • 標識
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • アプリケーション
    適用あり: ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant Rat ATPase Inhibitory Factor 1 (UniProtKB ID: Q6IAQ7).

  • ポジティブ・コントロール
    • ICC/IF: HepG2 cells.
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab198075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min).

ターゲット情報

  • 機能
    Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.
  • 配列類似性
    Belongs to the ATPase inhibitor family.
  • 翻訳後修飾
    Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.
  • 細胞内局在
    Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATIF1_HUMAN antibody
    • ATP synthase inhibitor protein antibody
    • ATPase inhibitor antibody
    • ATPase inhibitor mitochondrial antibody
    • ATPase inhibitor protein antibody
    • ATPase inhibitory factor 1 antibody
    • ATPI antibody
    • ATPIF 1 antibody
    • Atpif1 antibody
    • ATPIP antibody
    • IF(1) antibody
    • IF1 antibody
    • Inhibitor of F(1)F(o)-ATPase antibody
    • IP antibody
    • MGC1167 antibody
    • MGC8898 antibody
    • mitochondrial antibody
    see all

Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (Alexa Fluor® 488) 画像

  • ab198075 staining ATPase Inhibitory Factor 1 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198075 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ATPase Inhibitory Factor 1 antibody [5E2D7] (Alexa Fluor® 488) (ab198075) 使用論文

ab198075 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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