Anti-ATPB 抗体 [7E3F2] (ab110280)
Key features and details
- Mouse monoclonal [7E3F2] to ATPB
- Suitable for: WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ATPB antibody [7E3F2]
ATPB 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7E3F2] to ATPB -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Isolated mitochondria from Human heart, bovine heart, rat heart, mouse heart, and HepG2 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The purity of ab110280 is near homogeneity, as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
7E3F2 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110280の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa.
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特記事項 |
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WB
Use a concentration of 4 µg/ml. Predicted molecular weight: 57 kDa. |
ターゲット情報
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機能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. -
配列類似性
Belongs to the ATPase alpha/beta chains family. -
細胞内局在
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein. - Information by UniProt
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参照データベース
- Entrez Gene: 327675 Cow
- Entrez Gene: 506 Human
- Entrez Gene: 11947 Mouse
- Entrez Gene: 171374 Rat
- GenBank: BC016512 Human
- Omim: 102910 Human
- SwissProt: P00829 Cow
- SwissProt: P06576 Human
see all -
別名
- ATP 5B antibody
- ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
- ATP synthase subunit beta mitochondrial antibody
see all
画像
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All lanes : Anti-ATPB antibody [7E3F2] (ab110280) at 4 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 15 µg
Lane 2 : Isolated mitochondria from cow heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Lane 5 : Isolated mitochondria from HepG2 cells at 30 µg
Predicted band size: 57 kDa
データシートおよび資料
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Datasheet download
参考文献 (8)
ab110280 は 8 報の論文で使用されています。
- Yeom J & Groisman EA Reduced ATP-dependent proteolysis of functional proteins during nutrient limitation speeds the return of microbes to a growth state. Sci Signal 14:N/A (2021). PubMed: 33500334
- Yeom J & Groisman EA Low Cytoplasmic Magnesium Increases the Specificity of the Lon and ClpAP Proteases. J Bacteriol 203:e0014321 (2021). PubMed: 33941609
- Yeom J et al. Small proteins regulate Salmonella survival inside macrophages by controlling degradation of a magnesium transporter. Proc Natl Acad Sci U S A 117:20235-20243 (2020). PubMed: 32753384
- Hou X et al. Testosterone disruptor effect and gut microbiome perturbation in mice: Early life exposure to doxycycline. Chemosphere 222:722-731 (2019). PubMed: 30738315
- Park HE et al. Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio. Adv Sci (Weinh) 6:1901673 (2019). PubMed: 31763149
- Yeom J et al. Sequestration from Protease Adaptor Confers Differential Stability to Protease Substrate. Mol Cell 66:234-246.e5 (2017). PubMed: 28431231
- Yavlovich A et al. Ectopic ATP synthase facilitates transfer of HIV-1 from antigen-presenting cells to CD4(+) target cells. Blood 120:1246-53 (2012). PubMed: 22753871
- Kim M et al. Increased expression of the F(1)F(o) ATP synthase in response to iron in heart mitochondria. BMB Rep 41:153-7 (2008). WB ; Rat . PubMed: 18315952