Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15%)
Sample
Pig Cell lysate - whole cell (porcine retinal pigment epithelium (RPE) cells)
Specification
porcine retinal pigment epithelium (RPE) cells
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS/0.05% Tween
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 52 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
This antibody works also in the porcine samples, but the signal is much weaker in comparison to the human samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Jan 30 2015