Anti-ATPB 抗体 [3D5] - Mitochondrial Marker (HRP) (ab197905)

製品の概要

  • 製品名
    Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP)
    ATPB 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3D5] to ATPB - Mitochondrial Marker (HRP)
  • 標識
    HRP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Caenorhabditis elegans, Monkey
  • 免疫原

    Whole heart mitochondria (Human).

  • ポジティブ・コントロール
    • WB: Human heart mitochondrial lysate. HeLa and HepG2 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab197905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

ターゲット情報

  • 機能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
  • 配列類似性
    Belongs to the ATPase alpha/beta chains family.
  • 細胞内局在
    Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP 5B antibody
    • ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody
    • ATP synthase subunit beta mitochondrial antibody
    • ATP synthase subunit beta, mitochondrial antibody
    • atp5b antibody
    • ATPB antibody
    • ATPB_HUMAN antibody
    • ATPMB antibody
    • ATPSB antibody
    • Epididymis secretory protein Li 271 antibody
    • HEL-S-271 antibody
    • Mitochondrial ATP synthase beta subunit antibody
    • Mitochondrial ATP Synthase Subunit Beta antibody
    • Mitochondrial ATP synthetase beta subunit antibody
    see all

Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP) 画像

  • All lanes : Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP) (ab197905) at 1/5000 dilution

    Lane 1 : Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
    Lane 2 : HeLa whole cell lysate (ab150035) at 10 µg
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 2 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197905 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

プロトコール

Anti-ATPB antibody [3D5] - Mitochondrial Marker (HRP) (ab197905) 使用論文

ab197905 has not yet been referenced specifically in any publications.

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