Anti-ATP6V0A4 抗体 (ab97440)
Key features and details
- Rabbit polyclonal to ATP6V0A4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP6V0A4 antibody
ATP6V0A4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATP6V0A4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 53 and 265 of Human ATP6V0A4 (NP_570856)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97440の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 96 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 96 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
ATP6V0A4 (ATPase, H+ transporting, lysosomal V0 subunit a isoform 4) is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. -
細胞内局在
Apical cell membrane; Multi-pass membrane protein. -
参照データベース
- Entrez Gene: 50617 Human
- Entrez Gene: 140494 Mouse
- Omim: 605239 Human
- SwissProt: Q9HBG4 Human
- SwissProt: Q920R6 Mouse
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別名
- A4 antibody
- ATP6N1B antibody
- ATP6N2 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97440 は 3 報の論文で使用されています。
- Su K et al. Isoform a4 of the vacuolar ATPase a subunit promotes 4T1-12B breast cancer cell-dependent tumor growth and metastasis in vivo. J Biol Chem 298:102395 (2022). PubMed: 35988642
- McGuire CM et al. Isoform-specific gene disruptions reveal a role for the V-ATPase subunit a4 isoform in the invasiveness of 4T1-12B breast cancer cells. J Biol Chem 294:11248-11258 (2019). PubMed: 31167791
- Yanagisawa H et al. L-leucine and SPNS1 coordinately ameliorate dysfunction of autophagy in mouse and human Niemann-Pick type C disease. Sci Rep 7:15944 (2017). PubMed: 29162837