Anti-ATP5J 抗体 (ab224139)
Key features and details
- Rabbit polyclonal to ATP5J
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATP5J antibody -
製品の詳細
Rabbit polyclonal to ATP5J -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Cynomolgus monkey, Orangutan -
免疫原
Recombinant fragment corresponding to Human ATP5J aa 1 to the C-terminus.
Database link: P18859 -
ポジティブ・コントロール
- WB: RT4 and U-251 MG cell lysates; Human liver and tonsil tissue lysates. IHC-P: Human liver tissue. ICC/IF: U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab224139の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
特記事項 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
ターゲット情報
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機能
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. -
配列類似性
Belongs to the eukaryotic ATPase subunit F6 family. -
細胞内局在
Mitochondrion. Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 522 Human
- Omim: 603152 Human
- SwissProt: Q8SPH6 Cynomolgus monkey
- SwissProt: P18859 Human
- SwissProt: Q5RBY3 Orangutan
- Unigene: 246310 Human
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別名
- ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
- ATP synthase-coupling factor 6, mitochondrial antibody
see all
画像
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All lanes : Anti-ATP5J antibody (ab224139) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 13 kDa -
Paraffin-embedded human liver tissue stained for ATP5J using ab224139 at 1/500 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATP5J (green) using ab224139 at 4 µg/ml in ICC/IF.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab224139 は 2 報の論文で使用されています。
- Song W et al. Detection of mitochondrial coupling factor 6 in placental tissues from preeclamptic pregnancies and its influence on biological behavior of trophoblast cells. Exp Ther Med 22:1185 (2021). PubMed: 34475975
- Gale M et al. Acquired Resistance to HER2-Targeted Therapies Creates Vulnerability to ATP Synthase Inhibition. Cancer Res 80:524-535 (2020). PubMed: 31690671