Anti-ATP5H 抗体 [10G5AB9] (Alexa Fluor® 488) (ab198594)

製品の概要

  • 製品名
    Anti-ATP5H antibody [10G5AB9] (Alexa Fluor® 488)
    ATP5H 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10G5AB9] to ATP5H (Alexa Fluor® 488)
  • 標識
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • アプリケーション
    適用あり: ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, African green monkey
  • 免疫原

    Full length native protein (purified) corresponding to Cow ATP5H. Purified bovine OXPHOS complexes.

  • ポジティブ・コントロール
    • ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • 特記事項

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab198594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Flow Cyt 1/500.

ターゲット情報

  • 機能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.
  • 配列類似性
    Belongs to the ATPase d subunit family.
  • 細胞内局在
    Mitochondrion. Mitochondrion inner membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP synthase D chain mitochondrial antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit antibody
    • ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody
    • ATP synthase subunit d antibody
    • ATP synthase subunit d, mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody
    • ATP5H antibody
    • ATP5H_HUMAN antibody
    • ATP5JD antibody
    • ATPase subunit d antibody
    • ATPQ antibody
    • mitochondrial antibody
    • My032 protein antibody
    see all

Anti-ATP5H antibody [10G5AB9] (Alexa Fluor® 488) 画像

  • Overlay histogram showing HeLa cells stained with ab198594 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198594, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab198594 staining ATP5H in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198594 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-ATP5H antibody [10G5AB9] (Alexa Fluor® 488) (ab198594) 使用論文

ab198594 has not yet been referenced specifically in any publications.

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