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RabMAb

Anti-ATP5G1/G2/G3 抗体 [EPR13908] (ab180149)

製品の概要

  • 製品名
    Anti-ATP5G1/G2/G3 antibody [EPR13908]
  • 製品の詳細
    Rabbit monoclonal [EPR13908] to ATP5G1/G2/G3
  • アプリケーション
    適用あり: WB, IHC-P, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5G1/G2/G3 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P05496

  • ポジティブ・コントロール
    • HeLa and Human fetal heart lysates; Human liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab180149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 8 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/100.
IP 1/30.

画像

  • All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified)

    Lane 1 : mouse heart lysate
    Lane 2 : rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 14 kDa
    Observed band size : 8 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (purified) + human fetal heart lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 14 kDa
    Observed band size : 8 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human hepatocellular carcinoma with purified ab180149 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-ATP5G1/G2/G3 antibody [EPR13908] (ab180149) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size : 14 kDa
  • Immuohistochemical analysis of Paraffin-embedded Human liver tissue labeling ATP5G1 with unpurified ab180149 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.

  • Western blot analysis of fetal heart lysate immunoprecipitated with unpurified ab180149 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

参考文献

This product has been referenced in:
  • Alavian KN  et al. An uncoupling channel within the c-subunit ring of the F1FO ATP synthase is the mitochondrial permeability transition pore. Proc Natl Acad Sci U S A 111:10580-5 (2014). WB ; Human . Read more (PubMed: 24979777) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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