製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ATP5A
  • 特異性
    ab188107 is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Cow, Dog, Xenopus laevis, Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human ATP5A aa 83-94 (internal sequence) (Cysteine residue). (NP_004037.1).
    Sequence:

    RVHGLRNVQAEE


    Database link: P25705

  • ポジティブ・コントロール
    • WB: Human, mouse fetal, mouse, pig and rat heart lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab188107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 60, 54 kDa).

ターゲット情報

  • 機能
    Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
  • 組織特異性
    Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
  • 配列類似性
    Belongs to the ATPase alpha/beta chains family.
  • 翻訳後修飾
    The N-terminus is blocked.
  • 細胞内局在
    Mitochondrion inner membrane. Peripheral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATP synthase alpha chain, mitochondrial antibody
    • ATP synthase subunit alpha antibody
    • ATP synthase subunit alpha mitochondrial antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
    • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
    • ATP sythase (F1 ATPase) alpha subunit antibody
    • ATP5A antibody
    • Atp5a1 antibody
    • ATP5AL2 antibody
    • ATPA_HUMAN antibody
    • ATPM antibody
    • Epididymis secretory sperm binding protein Li 123m antibody
    • hATP1 antibody
    • HEL-S-123m antibody
    • MC5DN4 antibody
    • mitochondrial antibody
    • Mitochondrial ATP synthetase antibody
    • Mitochondrial ATP synthetase oligomycin resistant antibody
    • Modifier of Min 2 mouse homolog antibody
    • Modifier of Min 2, mouse, homolog of antibody
    • MOM2 antibody
    • OMR antibody
    • ORM antibody
    • OTTHUMP00000163475 antibody
    see all

画像

  • All lanes : Anti-ATP5A antibody (ab188107) at 0.01 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Fetal mouse heart lysate (in RIPA buffer)
    Lane 3 : Adult mouse heart lysate (in RIPA buffer)
    Lane 4 : Adult rat heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 60, 54 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • Anti-ATP5A antibody (ab188107) at 0.01 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 60, 54 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

プロトコール

参考文献

ab188107 has not yet been referenced specifically in any publications.

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