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RabMAb

Anti-ATP5A 抗体 [EPR13031] (ab178421)

製品の概要

  • 製品名
    Anti-ATP5A antibody [EPR13031]
    ATP5A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR13031] to ATP5A
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5A aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P25705

  • ポジティブ・コントロール
    • HeLa cells and cell lysates; HepG2 and Human fetal liver lysates; Human fetal heart tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab178421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.
    • 組織特異性
      Fetal lung, heart, liver, gut and kidney. Expressed at higher levels in the fetal brain, retina and spinal cord.
    • 配列類似性
      Belongs to the ATPase alpha/beta chains family.
    • 翻訳後修飾
      The N-terminus is blocked.
    • 細胞内局在
      Mitochondrion inner membrane. Peripheral membrane protein.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATP synthase alpha chain, mitochondrial antibody
      • ATP synthase subunit alpha antibody
      • ATP synthase subunit alpha mitochondrial antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, 1 antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle antibody
      • ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2 antibody
      • ATP sythase (F1 ATPase) alpha subunit antibody
      • ATP5A antibody
      • Atp5a1 antibody
      • ATP5AL2 antibody
      • ATPA_HUMAN antibody
      • ATPM antibody
      • Epididymis secretory sperm binding protein Li 123m antibody
      • hATP1 antibody
      • HEL-S-123m antibody
      • MC5DN4 antibody
      • mitochondrial antibody
      • Mitochondrial ATP synthetase antibody
      • Mitochondrial ATP synthetase oligomycin resistant antibody
      • Modifier of Min 2 mouse homolog antibody
      • Modifier of Min 2, mouse, homolog of antibody
      • MOM2 antibody
      • OMR antibody
      • ORM antibody
      • OTTHUMP00000163475 antibody
      see all

    画像

    • All lanes : Anti-ATP5A antibody [EPR13031] (ab178421) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : Human fetal liver lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 60 kDa
    • Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A with ab178421 at 1/50 dilution.

    • Immunofluorescent analysis of Hela cells labeling ATP5A with ab178421 at 1/100 dilution (red). DAPI nuclear staining (blue).

    参考文献

    ab178421 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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