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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ATP5A aa 200-300 (Cysteine residue). The exact sequence is proprietary. Isoform 1
Database link: P25705
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our Abpromise guarantee covers the use of ab176569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
The use of an HRP/AP polymerized secondary antibody is recommended.
|WB||1/1000 - 1/10000. Predicted molecular weight: 60 kDa.|
Immunofluorescence analysis of MCF7 cells labeling ATP5A using ab176569 at a 1/100 dilution (green). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATP5A using ab176569 at a 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human fetal heart tissue labeling ATP5A using ab176569 at a 1/50 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling ATP5A using ab176569 at a 1/10 dilution (red) or a rabbit IgG negative control (green).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"