Anti-ATP synthase Immunocapture 抗体 [12F4AD8AF8] (ab109867)
Key features and details
- Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-ATP synthase Immunocapture antibody [12F4AD8AF8] -
製品の詳細
Mouse monoclonal [12F4AD8AF8] to ATP synthase Immunocapture -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC or WB -
種交差性
交差種: Human -
免疫原
Full length protein. This information is considered to be commercially sensitive.
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ポジティブ・コントロール
- Bovine heart mitochondria ab110338
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特記事項
ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support.
This antibody was tested and confirmed not suitable for WB and ICC.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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特記事項(精製)
The purity of ab109867 is near homogeneity, as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation. -
一次抗体 備考
ab109867 is a sample of pure immunocapture antibody, not immobilized to a solid support. -
ポリ/モノ
モノクローナル -
クローン名
12F4AD8AF8 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109867の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
Complex V, also called F1F0ATPase or ATP synthase, is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclear encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.
画像
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Overlay histogram showing HepG2 cells stained with ab109867 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109867, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab109867 は 19 報の論文で使用されています。
- Chang YW et al. Quantitative phosphoproteomics reveals ectopic ATP synthase on mesenchymal stem cells to promote tumor progression via ERK/c-Fos pathway activation. Mol Cell Proteomics 21:100237 (2022). PubMed: 35439648
- Bahri H et al. TMEM70 forms oligomeric scaffolds within mitochondrial cristae promoting in situ assembly of mammalian ATP synthase proton channel. Biochim Biophys Acta Mol Cell Res 1868:118942 (2021). PubMed: 33359711
- Rao Y et al. Bouchardatine analogue alleviates non-alcoholic hepatic fatty liver disease/non-alcoholic steatohepatitis in high-fat fed mice by inhibiting ATP synthase activity. Br J Pharmacol 176:2877-2893 (2019). PubMed: 31113010
- Zhang M et al. SIRT5 deficiency suppresses mitochondrial ATP production and promotes AMPK activation in response to energy stress. PLoS One 14:e0211796 (2019). PubMed: 30759120
- Ni Z et al. AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Autophagy 14:685-701 (2018). PubMed: 29165041