Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary alveolar type II epithelial cells)
Specification
Primary alveolar type II epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2 saponin
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Dilution
1/300
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: 10% serum, 0.2% saponin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 488 Goat Anti-Mouse IgG (H+L) (2 mg/m
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
PubMed ID
22427691, 21844344: View
Additional Notes
also works well with MeOH fixation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 30 2012