Anti-ATG4D 抗体 (ab137621)
Key features and details
- Rabbit polyclonal to ATG4D
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ATG4D antibody
ATG4D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ATG4D -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Pig -
免疫原
Recombinant fragment corresponding to Human ATG4D aa 220-441.
Database link: Q86TL0 -
ポジティブ・コントロール
- IHC-P: SAS xenograft, mouse ovary tissue. ICC/IF: HEK-293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137621の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. -
組織特異性
Mainly expressed in skeletal muscle and, to a lower extent, in testis. -
配列類似性
Belongs to the peptidase C54 family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 84971 Human
- Entrez Gene: 235040 Mouse
- Entrez Gene: 100141400 Pig
- Entrez Gene: 686505 Rat
- Omim: 611340 Human
- SwissProt: Q86TL0 Human
- SwissProt: Q8BGV9 Mouse
- SwissProt: Q684M2 Pig
see all -
別名
- 9830134P12Rik antibody
- APG4 autophagy 4 homolog D antibody
- APG4 D antibody
see all
画像
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HEK-293T cells stained for ATG4D (green) using ab137621 at 1/500 dilution in ICC/IF.
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Paraffin embedded mouse ovary tissue stained for ATG4D using ab137621 at 1/500 dilution in immunohistochemical analysis.
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ab137621 at a 1/100 dilution, staining ATG4D in paraffin embedded SAS xenograft tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab137621 は 5 報の論文で使用されています。
- Del Bello B et al. Autophagy up-regulation by ulipristal acetate as a novel target mechanism in the treatment of uterine leiomyoma: an in vitro study. Fertil Steril 112:1150-1159 (2019). PubMed: 31843092
- Andaloussi AE et al. Defective expression of ATG4D abrogates autophagy and promotes growth in human uterine fibroids. Cell Death Discov 3:17041 (2017). PubMed: 28815060
- Zhang J et al. MECP2 expression in gastric cancer and its correlation with clinical pathological parameters. Medicine (Baltimore) 96:e7691 (2017). WB, IHC ; Human . PubMed: 28767600
- Blessing AM et al. Transcriptional regulation of core autophagy and lysosomal genes by the androgen receptor promotes prostate cancer progression. Autophagy 13:506-521 (2017). PubMed: 27977328
- Rothe K et al. The core autophagy protein ATG4B is a potential biomarker and therapeutic target in CML stem/progenitor cells. Blood 123:3622-34 (2014). WB ; Human . PubMed: 24755409