Anti-ATF-4 抗体 - C-terminal (ab1371)
Key features and details
- Goat polyclonal to ATF-4 - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-ATF-4 antibody - C-terminal
ATF-4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ATF-4 - C-terminal -
由来種
Goat -
特異性
This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human, Pig
交差が予測される動物種: Rat, Cow -
免疫原
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ポジティブ・コントロール
- WB: Mouse heart lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1371の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 37 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 37 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Transcriptional activator. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I. -
配列類似性
Belongs to the bZIP family.
Contains 1 bZIP domain. -
細胞内局在
Cytoplasm. Cell membrane. Nucleus. Colocalizes with GABBR1 in hippocampal neuron dendritic membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 509107 Cow
- Entrez Gene: 468 Human
- Entrez Gene: 11911 Mouse
- Entrez Gene: 79255 Rat
- Omim: 604064 Human
- SwissProt: Q3ZCH6 Cow
- SwissProt: P18848 Human
- SwissProt: Q06507 Mouse
see all -
別名
- Activating transcription factor 4 antibody
- ATF 4 antibody
- ATF4 antibody
see all
画像
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Anti-ATF-4 antibody - C-terminal (ab1371) at 1 µg/ml + Mouse Heart lysate at 35 µg
Predicted band size: 37 kDa
Observed band size: 35-37 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence. RIPA buffer used.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab1371 は 20 報の論文で使用されています。
- Xu R et al. The breast cancer oncogene IKKe coordinates mitochondrial function and serine metabolism. EMBO Rep 21:e48260 (2020). PubMed: 32783398
- Roberson PA et al. LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle. Front Nutr 7:628405 (2020). PubMed: 33521042
- Yi H et al. Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic Injury via Modulating PKC-ß/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways. Antioxid Redox Signal 30:1949-1968 (2019). PubMed: 30484323
- Zhao YS et al. Hydrogen and Oxygen Mixture to Improve Cardiac Dysfunction and Myocardial Pathological Changes Induced by Intermittent Hypoxia in Rats. Oxid Med Cell Longev 2019:7415212 (2019). PubMed: 30984338
- Huang T et al. Curcumin mitigates axonal injury and neuronal cell apoptosis through the PERK/Nrf2 signaling pathway following diffuse axonal injury. Neuroreport 29:661-677 (2018). PubMed: 29570500