Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
30 µg
Specification
kidney
Gel Running Conditions
Reduced Denaturing (4-12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Dilution
1/5000
Detection
Detection method
Fluorescence
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 15 2012