Anti-ARSA/ASA 抗体 [EPR11039] (ab174844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11039] to ARSA/ASA
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-ARSA/ASA antibody [EPR11039]
ARSA/ASA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11039] to ARSA/ASA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse非交差種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Human skin, A431 and HeLa lysates; Human colon and kidney tissues.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11039 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174844の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
1/1000 - 1/5000. Detects a band of approximately 60-62 kDa (predicted molecular weight: 54 kDa).
|
特記事項 |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000 - 1/5000. Detects a band of approximately 60-62 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
-
機能
Hydrolyzes cerebroside sulfate. -
関連疾患
Defects in ARSA are a cause of leukodystrophy metachromatic (MLD) [MIM:250100]. MLD is a disease due to a lysosomal storage defect. It is characterized by intralysosomal storage of cerebroside-3-sulfate in neural and non-neural tissues, with a diffuse loss of myelin in the central nervous system. Progressive demyelination causes a variety of neurological symptoms, including gait disturbances, ataxias, optical atrophy, dementia, seizures, and spastic tetraparesis. Three forms of the disease can be distinguished according to the age at onset: late-infantile, juvenile and adult.
Arylsulfatase A activity is defective in multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a disorder characterized by decreased activity of all known sulfatases. MSD is due to defects in SUMF1 resulting in the lack of post-translational modification of a highly conserved cysteine into 3-oxoalanine. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. -
配列類似性
Belongs to the sulfatase family. -
翻訳後修飾
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD). -
細胞内局在
Lysosome. - Information by UniProt
-
参照データベース
- Entrez Gene: 410 Human
- Entrez Gene: 11883 Mouse
- Omim: 607574 Human
- SwissProt: P15289 Human
- SwissProt: P50428 Mouse
- Unigene: 88251 Human
-
別名
- arsA antibody
- ARSA_HUMAN antibody
- arylsulfatase A antibody
see all
画像
-
All lanes : Anti-ARSA/ASA antibody [EPR11039] (ab174844) at 1/1000 dilution
Lane 1 : Human skin lysates
Lane 2 : A431 cell lysates
Lane 3 : HeLa cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling ARSA/ASA with ab174844 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ARSA/ASA with ab174844 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab174844 は 1 報の論文で使用されています。
- Yoo HS et al. Changes in plasma arylsulfatase A level as a compensatory biomarker of early Parkinson's disease. Sci Rep 10:5567 (2020). PubMed: 32221382