Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293)
Specification
HEK 293
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% BSA + 0.05% Triton-X100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor® 568 goat anti-rabbit IgG (H+L) highly
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/300
Additional data
Additional Notes
ARNT2 staining is in red and the nuclei are stained with DAPI (blue).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 03 2009