Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK cells)
Specification
MDCK cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5 % Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS containing 0.2 % BSA and 5 % serum
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Host species: Goat
Clonality: Polyclonal
Conjugation: Rhodamine
Dilution
1/200
Additional data
Additional Notes
Note: DISCOUNT CODE: 52823
This review is for the promotion of the above mentioned discount code.
MDCK cells were transfected with (A) empty vector or (B) vector encoding Myc-tagged human Nadrin. Immunofluorescence staining was performed with anti Nadrin (ab74454) and anti-Myc antibodies
Ab74454 antibody:
Exogenous human Nadrin expressed in MDCK cells was detected
Endogenous canine Nadrin in MDCK cells was not detected
This review is for the promotion of the above mentioned discount code.
MDCK cells were transfected with (A) empty vector or (B) vector encoding Myc-tagged human Nadrin. Immunofluorescence staining was performed with anti Nadrin (ab74454) and anti-Myc antibodies
Ab74454 antibody:
Exogenous human Nadrin expressed in MDCK cells was detected
Endogenous canine Nadrin in MDCK cells was not detected
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 28 2010