Anti-ARF1 抗体 (ab58578)
Key features and details
- Goat polyclonal to ARF1
- Suitable for: ELISA, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Dog, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ARF1 antibody
ARF1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ARF1 -
由来種
Goat -
アプリケーション
適用あり: ELISA, Flow Cyt, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Dog, Human
交差が予測される動物種: Xenopus laevis, Cynomolgus monkey -
免疫原
Synthetic peptide:
EGLDWLSNQLRNQK
by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1 -
ポジティブ・コントロール
- WB: A431, HeLa, HepG2, MDCK, NIH3T3 KNRK cell lysate. Flow Cyt: A431 cells
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特記事項
This antibody is expected to recognize all four reported isoforms.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
This antibody is expected to recognize all four reported isoforms. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58578の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 10 µg/ml.
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WB | (4) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. -
配列類似性
Belongs to the small GTPase superfamily. Arf family. -
細胞内局在
Golgi apparatus. Cytoplasm > perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 338058 Cow
- Entrez Gene: 100856517 Dog
- Entrez Gene: 375 Human
- Entrez Gene: 11840 Mouse
- Entrez Gene: 64310 Rat
- Entrez Gene: 380166 Xenopus laevis
- Omim: 103180 Human
- SwissProt: P84080 Cow
see all -
別名
- ADP Ribosylation Factor 1 antibody
- ADP-ribosylation factor 1 antibody
- ARF 1 antibody
see all
画像
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All lanes : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ARF1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab58578 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab58578 was shown to react with ARF1 in wild-type HeLa cells in Western blot with loss of signal observed in ARF1 knockout cell line ab264939 (ARF1 knockout cell lysate ab257353). Wild-type HeLa and ARF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58578 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse 680RD secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Lane 1 : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lanes 2-3 : Anti-ARF1 antibody (ab58578) at 0.03 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaLysates in RIPA buffer. Detected by chemiluminescence.
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All lanes : Anti-ARF1 antibody (ab58578) at 1 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : KNRK cell lysate
Lane 3 : MDCK cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaLysates in RIPA buffer. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab58578 は 11 報の論文で使用されています。
- Chui AJ et al. Activation of the CARD8 Inflammasome Requires a Disordered Region. Cell Rep 33:108264 (2020). PubMed: 33053349
- Mangleburg CG et al. Integrated analysis of the aging brain transcriptome and proteome in tauopathy. Mol Neurodegener 15:56 (2020). PubMed: 32993812
- Navare AT et al. Viral protein engagement of GBF1 induces host cell vulnerability through synthetic lethality. bioRxiv N/A:N/A (2020). PubMed: 33173868
- Steiner B et al. ER remodeling by the large GTPase atlastin promotes vacuolar growth of Legionella pneumophila. EMBO Rep 18:1817-1836 (2017). PubMed: 28835546
- Escoll P et al. Legionella pneumophila Modulates Mitochondrial Dynamics to Trigger Metabolic Repurposing of Infected Macrophages. Cell Host Microbe 22:302-316.e7 (2017). PubMed: 28867389