Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic stem cell)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
200000 cells
Specification
embryonic stem cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: rabbit anti-goat IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 250, 55, 42 kDa
Positive control
Lane 1
Additional data
Abcam response
Thank you for sharing this data with us. This antibody has previously been tested in western blot on mouse heart lysates, but it would appear from this abreview that it does not work on mouse ES cells, under these conditions. Abcam would welcome feedback from other customers who are using ab81972 for work on mouse cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 17 2010