製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to ARAP1
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Dog, Human, Pig
  • 免疫原

    Synthetic peptide: AFTADPLSLLRNV, corresponding to C terminal amino acids 1198-1210 of Human ARAP1.

  • 特記事項
    GenBank Accession Number – NP_056057; NP_631920


    The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. The RHO-GAP activity may mediate cell rounding and loss of stress fibers. At least three transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one variant has not been determined.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考
    The protein encoded by this gene contains ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. The RHO-GAP activity may mediate cell rounding and loss of stress fibers. At least three transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one variant has not been determined.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 19589776
WB Use at an assay dependent dilution. Detects a band of approximately 150 kDa (predicted molecular weight: 162 kDa).Can be blocked with Human ARAP1 peptide (ab23119). PubMed: 19589776

ターゲット情報

  • 機能
    Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
  • 組織特異性
    Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
  • 配列類似性
    Contains 1 Arf-GAP domain.
    Contains 4 PH domains.
    Contains 1 Ras-associating domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 細胞内局在
    Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2410002L19Rik antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARAP 1 antibody
    • Arap1 antibody
    • ARAP1_HUMAN antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1 antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1 antibody
    • Arf-GAP with Rho-GAP domain antibody
    • CENT D2 antibody
    • Centaurin delta 2 antibody
    • Centaurin delta2 antibody
    • Centaurin-delta-2 antibody
    • CENTD 2 antibody
    • CENTD2 antibody
    • Cnt d2 antibody
    • Cnt-d2 antibody
    • Cntd2 antibody
    • KIAA0782 antibody
    • MGC176140 antibody
    • mKIAA0782 antibody
    see all

画像

  • ab5912 staining ARAP1 in murine NIH3T3 cells by Immunocytochemistry/ Immunofluorescence.
  • Anti-ARAP1 antibody (ab5912) + lysate prepared from NIH3T3 cells

    Secondary
    HRP conjugated secondary

    Predicted band size : 162 kDa

    Image from Miyata M et al, J Biol Chem. 2009 Sep 4;284(36):24595-609. Epub 2009 Jul 9, Fig 7. DOI 10.1074/jbc.M109.016436

参考文献

This product has been referenced in:
  • Miyata M  et al. Regulation by afadin of cyclical activation and inactivation of Rap1, Rac1, and RhoA small G proteins at leading edges of moving NIH3T3 cells. J Biol Chem 284:24595-609 (2009). WB, ICC/IF ; Mouse . Read more (PubMed: 19589776) »

See 1 Publication for this product

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