Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemic Cancer Cell lines)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Specification
Leukemic Cancer Cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: ~52 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 31 2018