Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-APG5L/ATG5 antibody
APG5L/ATG5 抗体 (10件) 一覧
Description
Rabbit polyclonal to APG5L/ATG5
Tested applications
IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthetic peptide from the internal region of human, mouse and chicken APG5L/ATG5 conjugated to an immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cell Biology >> Apoptosis >> Phagocytosis
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
-
Immunohistochemistry (Frozen sections) - APG5L/ATG5 antibody (ab64227)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/300 - 1/2000.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/300 - 1/2000.
Target
Function
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.
May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Tissue specificity
Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Sequence similarities
Belongs to the ATG5 family.
Post-translational
modifications
Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
Cellular localization
Cytoplasm. Colocalizes with nonmuscle actin.
Target information above from: UniProt accessionQ9H1Y0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APG 5 antibody
- APG 5L antibody
- APG5 antibody
see all
Database links
- Entrez Gene: 9474 Human
- Entrez Gene: 11793 Mouse
- Omim: 604261 Human
- SwissProt: Q9H1Y0 Human
- SwissProt: Q99J83 Mouse
- Unigene: 486063 Human
- Unigene: 22264 Mouse
Anti-APG5L/ATG5 antibody images:
Immunohistochemistry (Frozen sections) - APG5L/ATG5 antibody (ab64227)
ab64227 staining mouse Testis tissue sections by immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at 20°C. The primary antibody was diluted 1/800 and incubated with sample for 20 hours at 20°C. The biotin conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary.
This image is courtesy of an Abreview submitted by Miss Silke Vorwald
References for Anti-APG5L/ATG5 antibody (ab64227)
ab64227 has not yet been referenced specifically in any publications.
Publishing research using ab64227? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"